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FISH过滤器 | 瑆创光学电子商店

FISH过滤器

荧光原位杂交(FISH)是指利用荧光染料标记的已知序列的单链核酸作为探针,通过碱基互补配对原理,与待检测样本中的核酸特异性结合,通过观察荧光信号对样本中待检测核酸进行定性、定量和定位分析。FISH以其速度快、信号强、特异性高、可多重染色等特点,广泛应用于产前诊断、基因组结构分析等领域。

 

影响 FISH 结果的因素有很多,包括样品制备和处理、显微镜、光源、荧光探针和滤光片。FISH 通常基于荧光显微镜来可视化信号,使用三种类型的滤光片:激发板、二向色镜和发射板。其工作原理如下图所示:

  • 激发 选择透过光源部分波长激发荧光探针
  •  光源发出反射样品透射产生荧光反射激发散射
  • 透射 选择荧光波段 某一部分透射 检测完全阻挡激发杂散
FISH实验滤波器带宽 大小FISH图像对比度重要影响增加带宽可以提高激发接收效率从而得到增强信号 实际接收信号包括探针荧光信号包括样品背景噪声带宽增加同时增加两者强度带宽降低降低背景噪声强度也会降低荧光信号
 
因此对于需要对比度FISH实验每种特定染料都会一个最佳带宽使FISH图像达到最佳对比度。Chroma带宽 FISH过滤器 针对常用FISH染料进行 优化您的FISH实验提供了最佳图像对比度
“ ”的信号“A 91 “ “ A ”噪音226 “ 对比度更好
Chroma FISH 滤光片具有以下特点
  • 透过率截止深度光谱
  • 提供最佳图像对比度同时保证足够亮度
  • 消除渗透影响
     a 消除渗漏绿色通道
     b ,去除金色橙色红色通道
  • 频带滤波器DAPI通道进行特殊处理避免DAPI信号干扰其他通道信号
  • 漂移多色图像叠加
  • 磁控 溅射 镀膜,终身保修。

ET系列-FISH多波段滤光片组

DAPI是一种能特异性结合DNA双链的有机荧光染料。在FISH实验中,DAPI主要用于对整个细胞核进行染色和成像,这导致DAPI的浓度比其他FISH探针高得多。在一些FISH实验过程中,DAPI染色在最后一步,染色后无需清洗步骤。

在使用多波段滤光片进行多色FISH实验中,为了避免DAPI的信号掩盖其他FISH探针的信号,Chroma公司的多波段滤光片组中激发板在DAPI处的透过率会刻意降低,以保证DAPI信号不会过高(见右图)。

Multi-band  FISH Filter set

DAPI 和 Aqua 的发射峰高度重叠,同时对两个探针进行成像通常很困难,因为大量的 DAPI 信号会掩盖 Aqua 信号。Chroma 的 69014 滤光片组通过降低 DAPI 的激发效率,可以同时观察 DAPI、Aqua、Green 和 Orange。DAPI 和 Aqua 使用不同的激发带,但共享相同的接收通道(见右图)。

69014 FISH Filter set

 
ET 系列 FISH 波段带通滤光片
光谱透射曲线  
滤片组 激发滤光片
(CWL/FWHM)nm
二向色镜
(50% cut-on)nm
发射机
(CWL/FWHM)nm
FISH 探针 备注
49000 350/50 400 460/50 DAPI  
49028 395/25 425 460/50 DAPI 适用于 395 nm 光源
49301 405/10 425 460/36 Blue  
49302 436/20 455 480/30 Aqua  
49303 495/25 515 537/29 Green 适合浅绿色且不含金色
49308 485/25 505 525/30 Green 适合金色且不含水
49312 490/20 505 525/20 Green 适合浅绿色且不含金色
49313 490/20 505 520/20 Green Ventanta FISH 或免疫荧光
49304 546/10 556 572/23 Gold  
49305 546/22 565 590/33 Orange 适合无红色
49309 539/21 556 576/31 Oange 适合红色
49306 580/25 600 625/30 Red 适合无金色和橙色
49310 592/21 610 630/30 Red 适合金色且不含橙色
49311 599/13 612 632/28 Red 适合橙色
49307 630/20 647 667/30 Far Red  
49009 640/30 660 690/50 Far Red  

ET系列FISH波段滤光片

光谱透射曲线  

滤片组 FISH 探针
DAPI Aqua Green Gold Orange Red Far Red
59001v2          
59002v2          
59010          
590111          
590121          
59033          
69008        
69011        
69013v2        
69014      
69015        
89084v22        
890853        
1 、为保证 绿色与橙色强度接近,59011适用于汞、卤素光源,59012适用于LED光源;​​​
2、包含一个双波段激发器(可同时激发绿色和橙色),一个三波段激发器(可同时激发DAPI、绿色和橙色)以及三个 单波段激发器( 可同时激发DAPI 、绿色和橙色);
3、包含一个可同时激发绿和红的双波段激发板,一个可同时激发DAPI、绿和红的三波段激发盘,以及三个分别激发DAPI 、绿和红的单波段激发盘。
*注:价格不包括任何运费、税费或清关费用

二向色分光镜(镜子)用作导光光学器件,用于反射指定范围的波长,并在指定的 AOI(通常为 45 度)处传输不同的指定范围的波长。Chroma  目录中的大多数二向色镜均适用于 45 度 AOI,如果不确定,请咨询我们。  二向色镜是落射荧光配置中使用的滤光片组的必要组件。

Chroma 制造 UltraFlat 二向色镜片,用于要求高水平表面平整度的激光应用,例如 TIRF 和其他超分辨率技术。我们提供 UltraFlat 二向色镜片,可用作单独部件或包含在组装的滤光片组中。

然而,平面二向色镜的秘诀在于它们需要在您的成像系统中保持平坦。使用夹子、弹簧或螺钉固定二向色镜的滤光块会使其失去平坦度。 Chroma 的 TIRF 和超分辨率滤光块 让您的二向色镜在显微镜中保持与我们制造时一样平坦。

我们的大多数二向色镜适用于更常规的宽场荧光应用,既没​​有也不需要高水平的表面平整度,但仍具有溅射干涉滤光片涂层的其他性能优势。

用于FISH波段带通滤光片组的二向色镜 

光谱透射曲线  

型号 波长 (CWL): nm  一系列滤光片:
T400lp 400 T400lp  属于以下系列:
49000 - ET/79001 - ET /49025 - ET
T425lpxr 425 T425lpxr  属于以下系列:
49021 - ET /49301 - ET /49028 - ET /49053 - ET 
T455lp 455 T455lp  属于以下系列:
49001 - ET /49052 - ET/49302 - ET/49032 - ET 
AT505DC 505 AT505DC  属于以下系列:
39002 - AT /39009 - AT /19002 - AT /19010 - AT
T515lp 515 T515lp  属于以下系列:
49003 - ET /49029 - ET/49303 - ET 
T556lpxr 556

T556lpxr  属于以下系列:49304 - ET/49309 - ET 

T565lpxr 565 T565lpxr  属于以下系列:
49004 - ET /49305 - ET 
AT600DC 600 AT600DC  属于以下系列:
39010 - AT /19006 - AT 
T610lpxr 610 T610lpxr  属于以下系列:49310 - ET
T612lpxr 612 T612lpxr  属于以下系列:49311 - ET
T647lpxr 647 T647lpxr  属于以下系列:49307 - ET 
T660lpxr 660 T660lpxr  属于以下系列:
49006 - ET/49009 - ET /49019 - ET 

*注:价格不包括任何运费、税费或清关费用


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Compare Model Drawings & Specs Availability Reference Price
(USD)
49000 Filter Set
49000 ET Series FISH Single Band Bandpass Filter Set,FISH Probe DAPI,Filters can be purchased separately
4-6 Weeks $725.00
49028 Filter Set
49028 ET Series FISH Single Band Bandpass Filter Set,FISH Probe DAPI,Filters can be purchased separately
4-6 Weeks $925.00
49301 Filter Set
49301 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Blue,Filters can be purchased separately
4-6 Weeks $925.00
49302 Filter Set
49302 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Aqua,Filters can be purchased separately
4-6 Weeks $925.00
49303 Filter Set
49303 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Green,Filters can be purchased separately
4-6 Weeks $925.00
49308 Filter Set
49308 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Green,Filters can be purchased separately
4-6 Weeks $925.00
49312 Filter Set
49312 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Green,Filters can be purchased separately
4-6 Weeks $925.00
49313 Filter Set
49313 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Green,Filters can be purchased separately
4-6 Weeks $925.00
49304 Filter Set
49304 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Gold,Filters can be purchased separately
4-6 Weeks $925.00
49305 Filter Set
49305 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Orange,Filters can be purchased separately
4-6 Weeks $925.00
49309 Filter Set
49309 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Oange,Filters can be purchased separately
4-6 Weeks $925.00
49306 Filter Set
49306 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Red,Filters can be purchased separately
4-6 Weeks $925.00
49310 Filter Set
49310 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Red,Filters can be purchased separately
4-6 Weeks $925.00
49311 Filter Set
49311 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Red,Filters can be purchased separately
4-6 Weeks $925.00
49307 Filter Set
49307 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Far Red,Filters can be purchased separately
4-6 Weeks $925.00
49009 Filter Set
49009 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Far Red,Filters can be purchased separately
4-6 Weeks $925.00
59001v2 Filter Set
59001v2 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green,Filters can be purchased separately
4-6 Weeks $1050.00
59002v2 Filter Set
59002v2 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Orange,Filters can be purchased separately
4-6 Weeks $1050.00
59010 Filter Set
59010 ET Series FISH Multi-band Filter Sets,FISH Probe Green/Red,Filters can be purchased separately
4-6 Weeks $1050.00
59011-1 Filter Set
59011-1 ET Series FISH Multi-band Filter Sets,FISH Probe Green/Orange,Filters can be purchased separately
4-6 Weeks $1050.00
59012-1 Filter Set
59012-1 ET Series FISH Multi-band Filter Sets,FISH Probe Green/Orange,Filters can be purchased separately
4-6 Weeks $1050.00
59033 Filter Set
59033 ET Series FISH Multi-band Filter Sets,FISH Probe Aqua/Gold,Filters can be purchased separately
4-6 Weeks $1050.00
69008 Filter Set
69008 ET Series FISH Multi-band Filter Sets,FISH Probe Aqua/Green/Red,Filters can be purchased separately
4-6 Weeks $1250.00
69011 Filter Set
69011 ET Series FISH Multi-band Filter Sets,FISH Probe Aqua/Green/Orange,Filters can be purchased separately
4-6 Weeks $1250.00
69013v2 Filter Set
69013v2 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green/Orange,Filters can be purchased separately
4-6 Weeks $1250.00
69014 Filter Set
69014 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Aqua/Green/Orange,Filters can be purchased separately
4-6 Weeks $1300.00
69015 Filter Set
69015 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green/Red,Filters can be purchased separately
4-6 Weeks $1250.00
89084v2-2 Filter Set
89084v2-2 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green/Orange,Filters can be purchased separately
4-6 Weeks $2350.00
89085-3 Filter Set
89085-3 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green/Red,Filters can be purchased separately
4-6 Weeks $2350.00
T610lpxr
T610lpxr Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 610 nm
4-6 Weeks $250.00
T612lpxr
T612lpxr Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 612 nm
4-6 Weeks $250.00
T647lpxr
T647lpxr Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 647 nm
4-6 Weeks $250.00
T556lpxr
T556lpxr Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 556 nm
4-6 Weeks $250.00
T425lpxr
Dichroic beamsplitters (mirrors),Wavelength (CWL): 425 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges
4-6 Weeks $250.00
T455lp
Dichroic beamsplitters (mirrors),Wavelength (CWL): 455 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges
4-6 Weeks $250.00
T400lp
T400lp Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 400 nm
4-6 Weeks $250.00
AT505DC
Dichroic beamsplitters (mirrors),Wavelength (CWL): 505 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges
4-6 Weeks $175.00
T515lp
Dichroic beamsplitters (mirrors),Wavelength (CWL): 515 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges
4-6 Weeks $250.00
T565lpxr
Dichroic beamsplitters (mirrors),Wavelength (CWL): 565 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges
4-6 Weeks $250.00
AT600DC
Dichroic beamsplitters (mirrors),Wavelength (CWL): 600 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges
4-6 Weeks $175.00

AT600DC - Parameter

T565lpxr - Parameter

T515lp - Parameter

AT505DC - Parameter

T455lp - Parameter

T400lp - Parameter

T556lpxr - Parameter

T425lpxr - Parameter

T647lpxr - Parameter

T612lpxr - Parameter

T610lpxr - Parameter

89085-3 Filter Set - Parameter

89084v2-2 Filter Set - Parameter

69015 Filter Set - Parameter

69014 Filter Set - Parameter

69013v2 Filter Set - Parameter

69011 Filter Set - Parameter

69008 Filter Set - Parameter

59033 Filter Set - Parameter

59012-1 Filter Set - Parameter

59011-1 Filter Set - Parameter

59010 Filter Set - Parameter

59002v2 Filter Set - Parameter

59001v2 Filter Set - Parameter

49009 Filter Set - Parameter

49307 Filter Set - Parameter

49311 Filter Set - Parameter

49310 Filter Set - Parameter

49306 Filter Set - Parameter

49309 Filter Set - Parameter

49305 Filter Set - Parameter

49304 Filter Set - Parameter

49313 Filter Set - Parameter

49312 Filter Set - Parameter

49308 Filter Set - Parameter

49303 Filter Set - Parameter

49302 Filter Set - Parameter

49301 Filter Set - Parameter

49028 Filter Set - Parameter

49000 Filter Set - Parameter

AT600DC - Download

T565lpxr - Download

T515lp - Download

AT505DC - Download

T455lp - Download

T400lp - Download

T556lpxr - Download

T425lpxr - Download

T647lpxr - Download

T612lpxr - Download

T610lpxr - Download

89085-3 Filter Set - Download

89084v2-2 Filter Set - Download

69015 Filter Set - Download

69014 Filter Set - Download

69013v2 Filter Set - Download

69011 Filter Set - Download

69008 Filter Set - Download

59033 Filter Set - Download

59012-1 Filter Set - Download

59011-1 Filter Set - Download

59010 Filter Set - Download

59002v2 Filter Set - Download

59001v2 Filter Set - Download

49009 Filter Set - Download

49307 Filter Set - Download

49311 Filter Set - Download

49310 Filter Set - Download

49306 Filter Set - Download

49309 Filter Set - Download

49305 Filter Set - Download

49304 Filter Set - Download

49313 Filter Set - Download

49312 Filter Set - Download

49308 Filter Set - Download

49303 Filter Set - Download

49302 Filter Set - Download

49301 Filter Set - Download

49028 Filter Set - Download

49000 Filter Set - Download

Accessories

Compare Model Drawings & Specs Availability Reference Price
(USD)