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Products / 光学元件 /滤光片 /TIRF显微镜过滤器 /

TIRF显微镜过滤器

产品概述规格配件联系下单！

对于多色TIRF，根据显微镜结构的不同，有两种不同的滤光片组配置。如果显微镜配有滤光片转轮，则可以将常规的多波段发射器（截止深度为OD6 - 7）加载到显微镜立方体中，可以将相应数量的单波段带通发射器（截止深度为OD6 - 7）加载到滤光片转轮中，最后一个波段可以与长通发射器一起使用。流道可以切换不同的带通发射器，以收集不同波长的荧光信号。将单波段带通发射器与多波段发射器匹配，可在相应的激光波长下提供 OD12+ 的截止深度。		基于物镜的TIRF光路图
对于没有滤光片转塔的显微镜，Chroma使用独特的工艺将两个相同的多波段发射器（截止深度为OD6 - 7）封装到金属外环（比传统滤光片厚）中，以实现OD8 - 10+的截止深度。		488 nm 激光单波段 TIRF 滤光片组
四波段 TIRF 滤光片组（无需滤光片轮）		四波段 TIRF 滤光片组（需要滤光片流道）

ET 系列 - TIRF 单波段带通滤光片组

光谱透射曲线

滤光片组	激发片 CWL/FWHM	二向色镜 RWL	带通发射机 CWL/FWHM	长通变送器50% 截止	激光波长（nm）
TRF49901	405/20	405	460/50	425	400 - 410
TRF49902	436/20	442	480/40	460	430 - 445
TRF49903	436/20	442	500/40	460	430 - 445
TRF49904	488/10	488	525/50	500	485 - 491
TRF49905	514/10	514	545/40	525	511 - 517
TRF49907	532/10	532	575/50	542	529 - 535
TRF49908	532/10	532	590/50	542	529 - 535
TRF49909	561/10	561	600/50	575	557 - 563
TRF49910	561/10	561	630/75	575	557 - 563
TRF49911	594/10	594	645/75	610	591 - 597
TRF49914	642/20	647	705/72	665	633 - 650

ET 系列 - TIRF 单波段长波通滤光片组

光谱透射曲线

滤光片组	激发片 CWL/FWHM	二向色镜 RWL	长发射器 - 1 50% 截止	长通发射器-2 50% 截止	激光波长（nm）
TRF49913	635/20	640	655	655	627 - 643

ET 系列 - TIRF 多波段带通滤光片组

光谱透射曲线

滤光片组	激光波长
滤光片组	405 纳米	442海里	488海里	514海里	532 纳米	561海里	594海里	640 纳米	647 纳米	需要跑步者
TRF59901	•		•							×
TRF59902		•		•						×
TRF59903		•			•					×
TRF59904			•			•				×
TRF59905			•				•			×
TRF59906			•					•		×
TRF59907					•			•		×
TRF69901	•		•			•				×
TRF69902	•		•				•			×
TRF69904		•		•		•				×
TRF69905		•		•			•			×
TRF89901v2	•		•			•		•		×
TRF89902	•		•			•			•	×
TRF59901-EM	•		•							√
TRF59902-EM		•		•						√
TRF59903-EM		•			•					√
TRF59904-EM			•			•				√
TRF59905-EM			•				•			√
TRF59906-EM			•					•		√
TRF59907-EM					•			•		√
TRF59908-EM1	•									√
TRF69901-EM	•		•			•				√
TRF69902-EM	•		•				•			√
TRF69904-EM		•		•		•				√
TRF69905-EM		•		•			•			√
TRF89901-EMv2	•		•			•		•		√
TRF89902-EM	•		•			•			•	√

*405 nm激光用于光活化

*注意：价格不包括任何运费、税费或清关费用

与传统的激光显微镜相比，全内反射荧光（TIRF）对二向色镜的平整度和厚度有更高的要求。平整度需要控制在0.5波长/英寸以内，二向色镜的厚度也必须达到2毫米或更厚，以避免在安装过程中外部应力对平整度的影响。

传统的显微镜立方体无法安装厚度为2毫米或更厚的二向色镜。此外，由于材料主要是塑料，抗变形能力也较差。针对TIRF应用，Chroma推出了一款专利保护的TIRF专用立方体（专利号：US8488238B2），具有以下特点：

能够安装厚度达3毫米的二向色镜；
能够安装最大厚度为6毫米的发射器，通过叠加两个发射器，允许在激光线及其附近增加OD值；
金属材料具有强大的抗变形能力，不会对安装的二向色镜造成应力。

立方体数	激发片	二向色镜	发射机	显微镜型号
91032	直径 25 mm	26 x 38 mm	直径 25 mm	尼康TE2000/Tea
	3.5 mm厚度（标准）	2mm厚度（标准）	6 mm 厚度（最大）
	6 mm 厚度（最大）	3mm 厚度（最大）
91041	直径 25 mm	26 x 38 mm	直径 25 mm	奥林巴斯IX71/IX81
	3.5 mm厚度（标准）	2mm厚度（标准）	6 mm 厚度（最大）
	6 mm 厚度（最大）	3mm 厚度（最大）
91044	直径 25 mm	26 x 38 mm	直径 25 mm	奥林巴斯IX73/IX83
	3.5 mm厚度（标准）	2mm厚度（标准）	6 mm 厚度（最大）
	6 mm 厚度（最大）	3mm 厚度（最大）
91042	直径 25 mm	25.5 x 36 mm	直径 25 mm	蔡司 Axio
	3.5 mm厚度（标准）	2mm厚度（标准）	6 mm 厚度（最大）
	6 mm 厚度（最大）	3mm 厚度（最大）

1、Chroma TIRF立方体不单独出售，需要Chroma 安装 TIRF二向色镜并校正其平坦度;

2、TIRF二向色镜用胶水固定。

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Search Reset

	Availability	Reference Price (USD)
TRF49902 Filter Set TRF49902 ET Series Single Band Bandpass Filter Set,Laser wavelength 430 - 445 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49901 Filter Set TRF49901 ET Series Single Band Bandpass Filter Set,Laser wavelength 400 - 410 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49903 Filter Set TRF49903 ET Series Single Band Bandpass Filter Set,Laser wavelength 430 - 445 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49904 Filter Set TRF49904 ET Series Single Band Bandpass Filter Set,Laser wavelength 485 - 491 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49905 Filter Set TRF49905 ET Series Single Band Bandpass Filter Set,Laser wavelength 511 - 517 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49907 Filter Set TRF49907 ET Series Single Band Bandpass Filter Set,Laser wavelength 529 - 535 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49908 Filter Set TRF49908 ET Series Single Band Bandpass Filter Set,Laser wavelength 529 - 535 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49909 Filter Set TRF49909 ET Series Single Band Bandpass Filter Set,Laser wavelength 557 - 563 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49910 Filter Set TRF49910 ET Series Single Band Bandpass Filter Set,Laser wavelength 557 - 563 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49911 Filter Set TRF49911 ET Series Single Band Bandpass Filter Set,Laser wavelength 591 - 597 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49914 Filter Set TRF49914 ET Series Single Band Bandpass Filter Set,Laser wavelength 633 - 650 nm,Filters can be purchased separately	4-6 Weeks	$1400.00
TRF49913 Filter Set TRF49913 ET Series TIRF Single Band Longpass Filter Set,Laser wavelength 627 - 643 nm,Filters can be purchased separately	4-6 Weeks	$1300.00
TRF59901 Filter Set TRF59901 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 488 nm,Filters can be purchased separately	4-6 Weeks	$1800.00
TRF59902 Filter Set TRF59902 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 442-514 nm,Filters can be purchased separately	4-6 Weeks	$1800.00
TRF59903 Filter Set TRF59903 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 442-532 nm,Filters can be purchased separately	4-6 Weeks	$1800.00
TRF59904 Filter Set TRF59904 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 488 - 561 nm,Filters can be purchased separately	4-6 Weeks	$1800.00
TRF59905 Filter Set TRF59905 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 488 - 594 nm,Filters can be purchased separately	4-6 Weeks	$1800.00
TRF59906 Filter Set TRF59906 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 488 -640 nm,Filters can be purchased separately	4-6 Weeks	$1800.00
TRF59907 Filter Set TRF59907 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 532 -640 nm,Filters can be purchased separately	4-6 Weeks	$1800.00
TRF69901 Filter Set TRF69901 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 561nm,Filters can be purchased separately	4-6 Weeks	$1900.00
TRF69902 Filter Set TRF69902 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 594 nm,Filters can be purchased separately	4-6 Weeks	$1900.00
TRF69904 Filter Set TRF69904 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 442 - 561nm,Filters can be purchased separately	4-6 Weeks	$1900.00
TRF69905 Filter Set TRF69905 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 442 - 594 nm,Filters can be purchased separately	4-6 Weeks	$1900.00
TRF89901v2 Filter Set TRF89901v2 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 640 nm,Filters can be purchased separately	4-6 Weeks	$2050.00
TRF89902 Filter Set TRF89902 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 647 nm,Filters can be purchased separately	4-6 Weeks	$2050.00
TRF59901-EM Filter Set TRF59901-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 488 nm,Filters can be purchased separately	4-6 Weeks	$2100.00
TRF59902-EM Filter Set TRF59902-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 442 - 514 nm,Filters can be purchased separately	4-6 Weeks	$2100.00
TRF59903-EM Filter Set TRF59903-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 442 - 532 nm,Filters can be purchased separately	4-6 Weeks	$2100.00
TRF59904-EM Filter Set TRF59904-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 488 - 561 nm,Filters can be purchased separately	4-6 Weeks	$2100.00
TRF59905-EM Filter Set TRF59905-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 488 - 594 nm,Filters can be purchased separately	4-6 Weeks	$2100.00
TRF59906-EM Filter Set TRF59906-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 488 - 640 nm,Filters can be purchased separately	4-6 Weeks	$2100.00
TRF59907-EM Filter Set TRF59907-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 532 - 640 nm,Filters can be purchased separately	4-6 Weeks	$2100.00
TRF59908-EM-1 Filter Set TRF59908-EM-1 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 nm,Filters can be purchased separately	4-6 Weeks	$2200.00
TRF69901-EM Filter Set TRF69901-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 561nm,Filters can be purchased separately	4-6 Weeks	$2550.00
TRF69902-EM Filter Set TRF69902-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 594 nm,Filters can be purchased separately	4-6 Weeks	$2550.00
TRF69904-EM Filter Set TRF69904-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 442 - 561 nm,Filters can be purchased separately	4-6 Weeks	$2550.00
TRF69905-EM Filter Set TRF69905-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 442 - 594 nm,Filters can be purchased separately	4-6 Weeks	$2550.00
TRF89901-EMv2 Filter Set TRF89901-EMv2 ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 640 nm,Filters can be purchased separately	4-6 Weeks	$2950.00
TRF89902-EM Filter Set TRF89902-EM ET Series TIRF Multi-Band Bandpass Filter Set,Laser wavelength 405 - 647 nm,Filters can be purchased separately	4-6 Weeks	$2950.00

91042 - Parameter

91044 - Parameter

91041 - Parameter

91032 - Parameter

TRF89902-EM Filter Set - Parameter

TRF89901-EMv2 Filter Set - Parameter

TRF69905-EM Filter Set - Parameter

TRF69904-EM Filter Set - Parameter

TRF69902-EM Filter Set - Parameter

TRF69901-EM Filter Set - Parameter

TRF59908-EM-1 Filter Set - Parameter

TRF59907-EM Filter Set - Parameter

TRF59906-EM Filter Set - Parameter

TRF59905-EM Filter Set - Parameter

TRF59904-EM Filter Set - Parameter

TRF59903-EM Filter Set - Parameter

TRF59902-EM Filter Set - Parameter

TRF59901-EM Filter Set - Parameter

TRF89902 Filter Set - Parameter

TRF89901v2 Filter Set - Parameter

TRF69905 Filter Set - Parameter

TRF69904 Filter Set - Parameter

TRF69902 Filter Set - Parameter

TRF69901 Filter Set - Parameter

TRF59907 Filter Set - Parameter

TRF59906 Filter Set - Parameter

TRF59905 Filter Set - Parameter

TRF59904 Filter Set - Parameter

TRF59903 Filter Set - Parameter

TRF59902 Filter Set - Parameter

TRF59901 Filter Set - Parameter

TRF49913 Filter Set - Parameter

TRF49914 Filter Set - Parameter

TRF49911 Filter Set - Parameter

TRF49910 Filter Set - Parameter

TRF49909 Filter Set - Parameter

TRF49908 Filter Set - Parameter

TRF49907 Filter Set - Parameter

TRF49905 Filter Set - Parameter

TRF49904 Filter Set - Parameter

TRF49903 Filter Set - Parameter

TRF49901 Filter Set - Parameter

TRF49902 Filter Set - Parameter

91042 - Download

91044 - Download

91041 - Download

91032 - Download

TRF89902-EM Filter Set - Download

TRF89901-EMv2 Filter Set - Download

TRF69905-EM Filter Set - Download

TRF69904-EM Filter Set - Download

TRF69902-EM Filter Set - Download

TRF69901-EM Filter Set - Download

TRF59908-EM-1 Filter Set - Download

TRF59907-EM Filter Set - Download

TRF59906-EM Filter Set - Download

TRF59905-EM Filter Set - Download

TRF59904-EM Filter Set - Download

TRF59903-EM Filter Set - Download

TRF59902-EM Filter Set - Download

TRF59901-EM Filter Set - Download

TRF89902 Filter Set - Download

TRF89901v2 Filter Set - Download

TRF69905 Filter Set - Download

TRF69904 Filter Set - Download

TRF69902 Filter Set - Download

TRF69901 Filter Set - Download

TRF59907 Filter Set - Download

TRF59906 Filter Set - Download

TRF59905 Filter Set - Download

TRF59904 Filter Set - Download

TRF59903 Filter Set - Download

TRF59902 Filter Set - Download

TRF59901 Filter Set - Download

TRF49913 Filter Set - Download

TRF49914 Filter Set - Download

TRF49911 Filter Set - Download

TRF49910 Filter Set - Download

TRF49909 Filter Set - Download

TRF49908 Filter Set - Download

TRF49907 Filter Set - Download

TRF49905 Filter Set - Download

TRF49904 Filter Set - Download

TRF49903 Filter Set - Download

TRF49901 Filter Set - Download

TRF49902 Filter Set - Download

Accessories

	Availability	Reference Price (USD)
91032 Laser TIRF for Nikon TE2000/Ti,Exciter (x) = 25mm, Emitter (m) = 25mm, Beamsplitter = 38x26mm	4-6 Weeks	Request for quote
91041 91041,Laser TIRF for Olympus BX2 Exciter (x) 25mm, Emitter (m) = 25mm, Beamsplitter = 26x36x2mm	4-6 Weeks	Request for quote
91044 91044,Laser TIRF for Olympus BX3/IX3 models, for 25mm filters,Exciter (x) = 25mm, Emitter (m) = 25mm, Beamsplitter = 26x36mm with a maximum thickness of 3mm	4-6 Weeks	Request for quote
91042 91042,Laser TIRF for Nikon TE2000/Ti,Exciter (x) = 25mm, Emitter (m) = 25mm, Beamsplitter = 38x26mm	4-6 Weeks	Request for quote